6OA3
Structure of human PARG complexed with JA2131
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-08-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.675, 65.948, 88.598 |
| Unit cell angles | 90.00, 95.44, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.18067 |
| Rwork | 0.178 |
| R-free | 0.22479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.771 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 37412 | 2326 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 20% PEG 3350, 0.05 M PCTP pH 7.5, 0.1 M NaCl, 0.15 M MgCl2 |
