6O9U
KirBac3.1 at a resolution of 2 Angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 105.955, 105.955, 89.639 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.608 - 2.000 |
| R-factor | 0.2034 |
| Rwork | 0.202 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xl4 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.610 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.111 | |
| Number of reflections | 65702 | 6508 |
| <I/σ(I)> | 14.97 | 1.24 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 2.5% PEG 4k, 2.5% PEG 8k, 10-17% PEG 400, 90 mM HEPES pH 7.5, 1 mM TCEP, and 50 mM EDTA |
