6O9I

Ternary complex of mouse ECD with Fab1 and Fab2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	CCD
Collection date	2016-02-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	41.430, 43.680, 133.820
Unit cell angles	87.86, 88.38, 81.17

Refinement procedure

Resolution	19.760 - 2.600
R-factor	0.2191
Rwork	0.216
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qkh
RMSD bond length	0.005
RMSD bond angle	0.803
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	PHENIX (dev-2328_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.760	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.192	0.967
Rmeas	0.216	1.087
Rpim	0.097	0.491
Total number of observations	137402	16771
Number of reflections	28394	3465
<I/σ(I)>	7.3	1.7
Completeness [%]	99.7	99.9
Redundancy	4.8	4.8
CC(1/2)	0.985	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Bis-Tris, pH 5.5, 0.2 M MgCl2 and 25% Peg3350