6O8C
Crystal structure of STING CTT in complex with TBK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2017-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 249.510, 249.510, 243.783 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.671 - 3.170 |
| R-factor | 0.2143 |
| Rwork | 0.213 |
| R-free | 0.23050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jlc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.086 |
| Data reduction software | iMOSFLM |
| Data scaling software | pointless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.671 | 3.240 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rmerge | 0.184 | 2.700 |
| Rpim | 0.054 | 0.850 |
| Number of reflections | 76001 | 4418 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.3 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 18% v/v tacsimate pH 7.0, 0.1 M HEPES pH 7.0, 2% PEG3350 |
