6O88
Crystal Structure of UDP-dependent glucosyltransferases (UGT) from Stevia rebaudiana in complex with UDP and rebaudioside A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 98.118, 98.118, 91.524 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.490 - 1.990 |
R-factor | 0.165 |
Rwork | 0.164 |
R-free | 0.20000 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.030 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.500 | 2.020 |
High resolution limit [Å] | 1.990 | 1.990 |
Rpim | 0.040 | |
Number of reflections | 35139 | 35139 |
<I/σ(I)> | 25.9 | |
Completeness [%] | 99.5 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 15% (w/v) PEG-4000, 20% 2-propanol (v/v), 100 mM sodium citrate tribasic dihydrate buffer pH 5.6 |