6O7Y

Trypanosoma cruzi EIF4E5 translation initiation factor in complex with cap-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9586
Spacegroup name	P 31 2 1
Unit cell lengths	102.590, 102.590, 41.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.350 - 2.200
R-factor	0.208
Rwork	0.207
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.200	2.200
Number of reflections	12900	2040
<I/σ(I)>	23.7
Completeness [%]	100.0
Redundancy	20.3
CC(1/2)	1.000	0.587

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.1M BisTris pH 6.5, 30% PEG3350, 0.2M Lithium Sulfate, 20% Glycerol