6O6Q

Crystal structure of Cka1p, a casein kinase 2 alpha ortholog from Candida albicans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-08-30
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	125.690, 125.690, 229.719
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.598 - 2.700
R-factor	0.2361
Rwork	0.234
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bqc
RMSD bond length	0.004
RMSD bond angle	0.647
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.710
High resolution limit [Å]	2.660	2.660
Rmerge	0.184	1.004
Rpim	0.043	0.305
Number of reflections	31301	1254
<I/σ(I)>	18.57	1.23
Completeness [%]	98.9	82.1
Redundancy	18.3	9.3
CC(1/2)		0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	2 M ammonium sulfate, 0.1 M sodium citrate pH 6.1, 1 mM AMP-PNP