6O64
Crystal Structure of Arabidopsis thaliana Spermidine Synthase isoform 2 (AtSPDS2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-01 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.646, 162.699, 97.494 |
Unit cell angles | 90.00, 100.21, 90.00 |
Refinement procedure
Resolution | 42.540 - 2.000 |
R-factor | 0.17659 |
Rwork | 0.176 |
R-free | 0.23052 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.855 |
Data reduction software | XDS (Version Jan 26, 2018) |
Data scaling software | STARANISO |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.557 |
Number of reflections | 125268 | 6263 |
<I/σ(I)> | 16.5 | 2.5 |
Completeness [%] | 97.1 | 97.1 |
Redundancy | 4.3 | 4.3 |
CC(1/2) | 0.999 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Morpheus screen G4 (Carboxylic acids, buffer system 1, precipitant mix 4) |