6O64
Crystal Structure of Arabidopsis thaliana Spermidine Synthase isoform 2 (AtSPDS2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.646, 162.699, 97.494 |
| Unit cell angles | 90.00, 100.21, 90.00 |
Refinement procedure
| Resolution | 42.540 - 2.000 |
| R-factor | 0.17659 |
| Rwork | 0.176 |
| R-free | 0.23052 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.855 |
| Data reduction software | XDS (Version Jan 26, 2018) |
| Data scaling software | STARANISO |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.557 |
| Number of reflections | 125268 | 6263 |
| <I/σ(I)> | 16.5 | 2.5 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.999 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | Morpheus screen G4 (Carboxylic acids, buffer system 1, precipitant mix 4) |
