6O4O

The structure of human interleukin 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95366
Spacegroup name	P 21 21 2
Unit cell lengths	39.020, 133.762, 27.183
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.459 - 1.620
R-factor	0.1775
Rwork	0.176
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mhl
RMSD bond length	0.010
RMSD bond angle	1.357
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.590	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.078
Rmeas	0.081
Rpim	0.023
Number of reflections	18978	918
<I/σ(I)>	16.2	2.3
Completeness [%]	100.0	99.9
Redundancy	12.9	13.7
CC(1/2)	0.999	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	18% PEG 3350, 0.1 M Bis-Tris propane pH 9, 0.2 M ammonium sulphate, 0.05 M praseodymium chloride, seeded