6O4J

Amyloid Beta KLVFFAENVGS 16-26 D23N Iowa mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ELECTRON MICROSCOPE
Collection date	2014-11-07
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	11.670, 51.910, 12.760
Unit cell angles	90.00, 114.18, 90.00

Refinement procedure

Resolution	11.640 - 1.402
R-factor	0.2419
Rwork	0.237
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y2a
RMSD bond length	0.014
RMSD bond angle	1.458
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	11.640	11.640	1.440
High resolution limit [Å]	1.400	6.270	1.400
Rmerge	0.240	0.298	0.652
Rmeas	0.246	0.308	0.682
Total number of observations	47598
Number of reflections	2355	25	163
<I/σ(I)>	9.06	11.44	2.88
Completeness [%]	85.4	86.2	78
Redundancy	20.211	13.32	12.061
CC(1/2)	0.995	0.987	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	UNSPECIFIED