6O42

Crystal structure of the germline PGZL1 (PGZL1_gVmDmJ) Fab in complex with MPER peptide epitope.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	208.360, 37.920, 133.520
Unit cell angles	90.00, 93.68, 90.00

Refinement procedure

Resolution	39.766 - 2.600
R-factor	0.2593
Rwork	0.257
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6o41
RMSD bond length	0.002
RMSD bond angle	0.536
Data reduction software	XDS (20180319)
Data scaling software	XSCALE (20180319)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.770	2.670
High resolution limit [Å]	2.600	2.600
Rpim	0.107	0.731
Number of reflections	30972	2317
<I/σ(I)>	6.4	1.2
Completeness [%]	93.8	95.2
Redundancy	2.9	2.8
CC(1/2)	0.987	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293.15	0.1 M sodium acetate pH 4.6, 0.2 M ammonium sulfate, 30% PEG 2000 monomethyl ether, 8 MM 06:0 PA