6O3O

Structure of human DNAM-1 (CD226) bound to nectin-like protein-5 (necl-5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	129.337, 182.269, 146.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.990 - 2.800
R-factor	0.209
Rwork	0.208
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r0n 4fqp
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.500	2.950
High resolution limit [Å]	2.800	2.800
Rpim	0.094	0.470
Number of reflections	42723	6171
<I/σ(I)>	7.9	1.9
Completeness [%]	100.0	100
Redundancy	19.7	19.8
CC(1/2)	0.987	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG3350, 0.18M K2SO4, 10mM EDTA