Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.685, 57.450, 86.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.842 - 1.700 |
| R-factor | 0.1719 |
| Rwork | 0.170 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2anw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.735 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.032 | 0.339 |
| Number of reflections | 31089 | |
| <I/σ(I)> | 16 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.9 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 24% PEG 3350, 0.2M potassium dihydrogen phosphate |
