6NZ7

Crystal structure of broadly neutralizing Influenza A antibody 429 B01 in complex with Hemagglutinin Hong Kong 1968

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	91.769, 123.905, 119.338
Unit cell angles	90.00, 112.00, 90.00

Refinement procedure

Resolution	42.225 - 2.950
R-factor	0.2258
Rwork	0.224
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.950	8.000	2.950
Rmerge	0.105	0.052	0.509
Rmeas	0.126	0.063	0.661
Rpim	0.069	0.036	0.417
Number of reflections	49672	2542	1543
<I/σ(I)>	8.5
Completeness [%]	94.7	93.8	59.3
Redundancy	3.1	3	1.7
CC(1/2)		0.993	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	300	10 % PEG8000 (w/v), 100 mM Tris/Cl, pH 8.5 and 7.5 % 1,6-hexanediol (w/v)