6NWV
Insulin Lispro Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.161, 61.789, 58.618 |
| Unit cell angles | 90.00, 111.46, 90.00 |
Refinement procedure
| Resolution | 42.960 - 1.601 |
| R-factor | 0.21318 |
| Rwork | 0.208 |
| R-free | 0.25673 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ev6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.409 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.960 | 1.690 |
| High resolution limit [Å] | 1.601 | 1.600 |
| Rmerge | 0.101 | 1.856 |
| Rpim | 0.042 | 0.781 |
| Number of reflections | 40488 | 4189 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.375 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M HEPES sodium pH 7.5; 0.4 M Potassium sodium tartrate tetrahydrate. |
