6NWU

RORgamma Ligand Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	59.880, 59.880, 162.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.189 - 3.200
R-factor	0.2237
Rwork	0.218
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vb3
RMSD bond length	0.003
RMSD bond angle	0.677
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.190	3.300
High resolution limit [Å]	3.200	3.200
Number of reflections	5345
<I/σ(I)>	9.55
Completeness [%]	100.0
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	0.1 M sodium phosphate, pH 7.4, 0.5-0.7 M sodium chloride, 4-6% PEG4000, 3% DMSO