6NTZ
Crystal structure of E. coli PBP5-meropenem
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-09 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.810, 50.800, 80.220 |
Unit cell angles | 90.00, 118.69, 90.00 |
Refinement procedure
Resolution | 46.082 - 2.200 |
R-factor | 0.2161 |
Rwork | 0.214 |
R-free | 0.26190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mzf |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.082 | 46.082 | 2.390 |
High resolution limit [Å] | 2.200 | 10.420 | 2.330 |
Rmerge | 0.127 | 0.025 | 6.200 |
Rmeas | 0.140 | 0.027 | 6.986 |
Number of reflections | 52513 | 694 | 3703 |
<I/σ(I)> | 7.27 | 34.38 | 0.24 |
Completeness [%] | 99.5 | 97.9 | 96.7 |
Redundancy | 5.725 | 5.305 | 4.475 |
CC(1/2) | 0.999 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298.15 | PBP5 protein was crystallized using 0.2 uL protein solution (5 mg/mL purified protein in 20 mM HEPES pH8, 300 mM NaCl) and 1 uL of mother liquor (0.1 M tris pH 7, 7% PEG 400) with the addition of 1 mM meropenem. |