6NTP
PTP1B Domain of PTP1B-LOV2 Chimera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99994 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 89.254, 89.254, 105.747 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.670 - 1.890 |
R-factor | 0.18073 |
Rwork | 0.179 |
R-free | 0.21175 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a5j |
RMSD bond length | 0.008 |
RMSD bond angle | 1.488 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.870 | 1.920 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.071 | 0.071 |
Rmeas | 0.075 | |
Rpim | 0.025 | |
Number of reflections | 39530 | 1936 |
<I/σ(I)> | 17.2 | 9.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM HEPES, 200 mM magnesium acetate, and 14% polyethylene glycol 8000, pH 7.5 |