6NTD
Crystal Structure of G12V HRas-GppNHp bound in complex with the engineered RBD variant 12 of CRAF Kinase protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 91.761, 91.761, 151.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.470 - 3.150 |
| R-factor | 0.2737 |
| Rwork | 0.273 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g0n |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.470 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.147 | |
| Number of reflections | 7192 | 1164 |
| <I/σ(I)> | 11 | 0.4 |
| Completeness [%] | 97.9 | 90 |
| Redundancy | 12.3 | 12.6 |
| CC(1/2) | 0.998 | 0.439 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 30% PEG4000, 200mM NH4SO4, 100mM Na Citrate |
