6NRW

Crystal structure of Dpr1 IG1 bound to DIP-eta IG1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2015-03-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 43 21 2
Unit cell lengths	74.084, 74.084, 235.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.570 - 2.400
R-factor	0.2138
Rwork	0.212
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eo9
RMSD bond length	0.004
RMSD bond angle	0.995
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX ((dev_3112: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.140	0.664
Rmeas	0.145	0.732
Number of reflections	25092	724
<I/σ(I)>	1.8
Completeness [%]	93.4	55.7
Redundancy	13.6	4.7
CC(1/2)		0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	294	0.2 M lithium sulfate, 0.1 M MES, pH 6, 20% (w/v) PEG 4000