6NPM
Crystal structure of Epstein-Barr Virus Nuclear Antigen-1, EBNA1, bound to fragments
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.458, 67.951, 69.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.955 - 1.603 |
| R-factor | 0.1446 |
| Rwork | 0.143 |
| R-free | 0.16960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vhi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.955 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 37306 | 2574 |
| <I/σ(I)> | 41.7 | 2.5 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 50 mM MES pH 6.5, 0-100 mM NaCl, 10 mM DTT |
