6NP9
PD-L1 IgV domain V76T with fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.331, 54.111, 85.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.427 - 1.270 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bik |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.895 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.055 | 0.288 |
| Rpim | 0.018 | 0.112 |
| Number of reflections | 32928 | 1604 |
| <I/σ(I)> | 61 | 7.1 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 9.2 | 7.1 |
| CC(1/2) | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | Ammonium Sulfate |
