6NOJ

PD-L1 IgV domain V76T with fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	83.803, 96.941, 32.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.084 - 2.330
R-factor	0.1956
Rwork	0.190
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bik
RMSD bond length	0.011
RMSD bond angle	1.259
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.390
High resolution limit [Å]	2.330	2.330
Rmerge	0.099	0.505
Rmeas	0.159	0.539
Rpim	0.051	0.183
Number of reflections	16612	803
<I/σ(I)>	11.3
Completeness [%]	99.5	99.9
Redundancy	8.8	7.9
CC(1/2)		0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	0.056 M sodium phosphate monobasic, 1.344 M potassium phosphate dibasic