6NMT
Non-Blocking Fab 3 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 126.370, 126.370, 229.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.190 - 1.830 |
| R-factor | 0.1789 |
| Rwork | 0.178 |
| R-free | 0.20380 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.190 | 1.850 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 81726 | 5960 |
| <I/σ(I)> | 15.2 | 0.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 285 | 0.1 M Sodium Acetate, 0.45 M Ammonium Sulfate, 35% w/v PEG 4000 |
