6NL7
Crystal structure of B1 immunoglobulin-binding domain of Streptococcal Protein G (T16F, T18A, V21H, T25H, K28Y, V29I, K31R, Q32A, Y33L, N35K, D36A, N37Q)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.098 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.903, 63.057, 53.225 |
Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
Resolution | 40.060 - 1.400 |
R-factor | 0.11511 |
Rwork | 0.113 |
R-free | 0.15813 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pga |
RMSD bond length | 0.016 |
RMSD bond angle | 1.850 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.800 | 1.434 |
High resolution limit [Å] | 1.400 | 1.398 |
Rmerge | 0.100 | |
Number of reflections | 44547 | 932 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 90.0 | 25.89 |
Redundancy | 9.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 80 mM acetic acid pH 3.6, 20 mM acetic acid pH 5.8, 30% 2,4-methylpentanediol, 200 mM NaCl and 20 mM zinc sulfate |