6NIF

crystal structure of human REV7-RAN complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	64.571, 64.571, 113.775
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.285 - 2.002
R-factor	0.2285
Rwork	0.226
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3abd
RMSD bond length	0.007
RMSD bond angle	0.838
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.285	2.030
High resolution limit [Å]	2.002	2.002
Rmeas	0.086	0.325
Rpim	0.020	0.076
Number of reflections	18976	934
<I/σ(I)>	41.7
Completeness [%]	99.2	97.16
Redundancy	19.2	18.3
CC(1/2)		0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	100 mM HEPES, pH 7.0, 1.1 M sodium malonate, 0.5% Jeffamine ED-2001