6NFP
1.7 Angstrom Resolution Crystal Structure of Arginase from Bacillus subtilis subsp. subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.414, 143.989, 85.515 |
| Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
| Resolution | 29.990 - 1.700 |
| R-factor | 0.1408 |
| Rwork | 0.139 |
| R-free | 0.16646 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cev |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.373 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.061 | 0.779 |
| Rmeas | 0.069 | 0.887 |
| Rpim | 0.032 | 0.421 |
| Number of reflections | 191464 | 9654 |
| <I/σ(I)> | 20.3 | 2.2 |
| Completeness [%] | 94.5 | 95.3 |
| Redundancy | 4.4 | 4.4 |
| CC(1/2) | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | Protein: 40.0 mg/ml, 0.3M Sodium chloride, 0.01M HEPES pH 7.5, Screen: 0.2M Magnesium chloride,0.1M Tris-HCL pH 8.5, 17% (w/v) PEG 3350 |
