6NFK

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B with loop 7 from APOBEC3G bound to iodide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	50.600, 50.600, 149.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.780 - 1.860
R-factor	0.1914
Rwork	0.189
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqd
RMSD bond length	0.015
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_3366: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.800	1.920
High resolution limit [Å]	1.860	1.860
Rmerge	0.057	1.300
Rmeas	0.062	1.410
Rpim	0.024	0.540
Number of reflections	17023	1631
<I/σ(I)>	14	1.4
Completeness [%]	99.4	98.43
Redundancy	6.5	6.4
CC(1/2)	0.999	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium Iodide, PEG3350