6NFF

Structure of X-prolyl dipeptidyl aminopeptidase from Lactobacillus helveticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION NOVA
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-29
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	154.630, 154.630, 106.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.300 - 2.000
R-factor	0.2382
Rwork	0.236
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lns
RMSD bond length	0.007
RMSD bond angle	0.874
Data reduction software	CrysalisPro
Data scaling software	Aimless
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.300	2.040
High resolution limit [Å]	2.000	2.000
Rmeas	0.401	4.952
Number of reflections	87344	4411
<I/σ(I)>	8.1	0.5
Completeness [%]	100.0	100
Redundancy	18.3	10.4
CC(1/2)	0.987	0.249

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	5.3 mg/mL protein against 6.8-8.2% PEG3350, 75 mM potassium phosphate, pH 6.0, 1 mM DTT