6NFF
Structure of X-prolyl dipeptidyl aminopeptidase from Lactobacillus helveticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION NOVA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-29 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 154.630, 154.630, 106.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.300 - 2.000 |
R-factor | 0.2382 |
Rwork | 0.236 |
R-free | 0.27290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lns |
RMSD bond length | 0.007 |
RMSD bond angle | 0.874 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.300 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.401 | 4.952 |
Number of reflections | 87344 | 4411 |
<I/σ(I)> | 8.1 | 0.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.3 | 10.4 |
CC(1/2) | 0.987 | 0.249 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 5.3 mg/mL protein against 6.8-8.2% PEG3350, 75 mM potassium phosphate, pH 6.0, 1 mM DTT |