6NDJ

Crystal structure of human NLRP6 PYD domain with MBP fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	38.283, 65.364, 102.362
Unit cell angles	95.63, 92.13, 106.59

Refinement procedure

Resolution	101.630 - 2.270
R-factor	0.1895
Rwork	0.187
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.493
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.630	2.440
High resolution limit [Å]	2.270	2.270
Number of reflections	43656
<I/σ(I)>	10.5
Completeness [%]	92.3
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, 25% PEG3350