6NCW
Crystal structure of a GH2 beta-galacturonidase from Eisenbergiella tayi bound to glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.930, 87.677, 124.375 |
| Unit cell angles | 93.29, 101.55, 90.37 |
Refinement procedure
| Resolution | 29.542 - 2.100 |
| R-factor | 0.1654 |
| Rwork | 0.165 |
| R-free | 0.20770 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.927 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.542 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.093 | 0.509 |
| Number of reflections | 118452 | 11739 |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 97.9 | 97 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.995 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M NaH2PO4/K2HPO4, pH 8.2 |
