6NCU
Interleukin-37 residues 53-206- dimer
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-07-09 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000030 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.510, 67.510, 142.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.103 - 3.500 |
R-factor | 0.3463 |
Rwork | 0.340 |
R-free | 0.39880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hn1 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3228) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.103 | 3.852 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.090 | 4.555 |
Number of reflections | 5177 | 1382 |
<I/σ(I)> | 16.6 | |
Completeness [%] | 99.8 | |
Redundancy | 19.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | JCSG Core I (Qiagen) screen, condition 92: 0.1M Citric acid pH 2.5, 20%MPD |