6NBW
Ternary Complex of Beta/Gamma-Actin with Profilin and AnCoA-NAA80
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9775 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 104.406, 115.594, 132.195 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.100 - 2.500 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pbd |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.496 | 2.500 |
| Rmerge | 0.116 | 0.563 |
| Number of reflections | 28132 | 2785 |
| <I/σ(I)> | 21 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 15% PEG 3350, 50mM MES pH6.2, 50mM NH4NO3 |
