6NAX
Olfactomedin domain of mouse myocilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2017-02-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 4 |
Unit cell lengths | 111.955, 111.955, 44.129 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.100 - 1.551 |
R-factor | 0.1582 |
Rwork | 0.158 |
R-free | 0.17790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wxq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.873 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((1.11.1_2575)) |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.100 | 1.607 |
High resolution limit [Å] | 1.550 | 1.551 |
Rmerge | 0.067 | 0.210 |
Rmeas | 0.076 | 0.242 |
Rpim | 0.035 | 0.118 |
Number of reflections | 79283 | 7499 |
<I/σ(I)> | 18.04 | 6.06 |
Completeness [%] | 98.5 | 95.2 |
Redundancy | 4.5 | 3.9 |
CC(1/2) | 0.996 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1:1 drop ratio of 30 mg/mL mouse OLF in 10 mM Na/K Phosphate pH 7.2 and mother liquor comprising 10% PEG 8000, 200 mM magnesium chloride |