6NAQ
Crystal structure of Neisseria meningitidis ClpP protease in Apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2016-01-15 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.23894 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.584, 127.945, 120.195 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 51.390 - 2.022 |
| R-factor | 0.2088 |
| Rwork | 0.207 |
| R-free | 0.24590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dkp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.390 | 2.150 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.097 | 0.290 |
| Number of reflections | 190314 | 5570 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.996 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.2 M potassium thiocyanate 40% MPD |
