6N9E

Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution

This is a large structure.

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (3.7 Å)

Cell axes212.240452.840620.300
Cell angles90.0090.0090.00
SpacegroupP 21 21 21
Resolution limits146.71 - 3.70
the highest resolution shell value3.742 - 3.700
R-factor0.2388
R-work0.23670
the highest resolution shell value0.339
R-free0.27770
the highest resolution shell value0.366
RMSD bond length0.003
RMSD bond angle0.635

Data Collection Statistics

Resolution limits146.71 - 3.70
the highest resolution shell value -
Number of reflections627226
Rmerge_l_obs0.237
the highest resolution shell value2.508
Completeness99.4
Redundancy8.655
the highest resolution shell value7.946
I/sigma(I)-3

Crystallization Conditions

crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION7.62920.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*
170383
PDB entries from 2020-10-28