6N9E
Crystal structure of the Thermus thermophilus 70S ribosome in complex with a short substrate mimic CC-Pmn and bound to mRNA and P-site tRNA at 3.7A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 212.240, 452.840, 620.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 146.711 - 3.700 |
R-factor | 0.2388 |
Rwork | 0.237 |
R-free | 0.27770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y4p |
RMSD bond length | 0.003 |
RMSD bond angle | 0.635 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 146.711 | 146.711 | 3.800 |
High resolution limit [Å] | 3.700 | 16.550 | 3.700 |
Rmerge | 0.237 | 0.048 | 2.508 |
Rmeas | 0.253 | 0.052 | 2.690 |
Total number of observations | 5428817 | ||
Number of reflections | 627226 | 7395 | 45894 |
<I/σ(I)> | 6.87 | 39.25 | 0.88 |
Completeness [%] | 99.4 | 97.2 | 99 |
Redundancy | 8.655 | 8.919 | 7.946 |
CC(1/2) | 0.997 | 0.997 | 0.188 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |