6N8S
Crystal structure of the human cell polarity protein Lethal Giant Larvae 2 (Lgl2). aPKC phosphorylated, crystal form 3.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 130.871, 130.871, 277.649 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.660 - 3.900 |
R-factor | 0.2556 |
Rwork | 0.252 |
R-free | 0.29780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.453 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.660 | 4.210 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 1.246 | 4.841 |
Number of reflections | 22815 | 4624 |
<I/σ(I)> | 4 | 1.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.1 | 13.8 |
CC(1/2) | 0.953 | 0.416 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295.15 | 1.4 M ammonium sulfate, 100 mM Hepes pH 7.5, 150 mM NaCl, 1.07% 1,6-hexanediol |