6N8R

Crystal structure of the human cell polarity protein Lethal Giant Larvae 2 (Lgl2). aPKC phosphorylated, crystal form 2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	173.570, 46.562, 137.228
Unit cell angles	90.00, 115.84, 90.00

Refinement procedure

Resolution	34.272 - 1.910
R-factor	0.1973
Rwork	0.196
R-free	0.21920
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.050	1.950
High resolution limit [Å]	1.910	1.910
Rmerge	0.111	10.548
Rmeas	0.121	11.922
Rpim	0.047	5.476
Total number of observations	429153
Number of reflections	66987	2420
<I/σ(I)>	7.9
Completeness [%]	86.9	47.1
Redundancy	6.4	4.6
CC(1/2)	0.999	0.117

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295.15	25% PEG 1500, 100 mM SPG pH 8.0