6N8Q

Crystal structure of the human cell polarity protein Lethal Giant Larvae 2 (Lgl2). Unphosphorylated, crystal form 2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0055
Spacegroup name	C 1 2 1
Unit cell lengths	174.528, 46.759, 137.689
Unit cell angles	90.00, 116.00, 90.00

Refinement procedure

Resolution	39.216 - 2.200
R-factor	0.2074
Rwork	0.206
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.220	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.112	3.398
Rmeas	0.125	3.776
Rpim	0.054	1.610
Number of reflections	48731	3566
<I/σ(I)>	7.8
Completeness [%]	95.3	81
Redundancy	5.4	5.2
CC(1/2)	0.998	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295.15	18-21 % PEG 2000 MME, 80-100 mM SPG pH 6.0