6N8D
Crystal structure of GII.4 2002 norovirus P domain in complex with neutralizing human antibody A1431
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.369, 157.356, 110.362 |
| Unit cell angles | 90.00, 112.93, 90.00 |
Refinement procedure
| Resolution | 41.622 - 3.100 |
| R-factor | 0.1787 |
| Rwork | 0.176 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oov 3eyq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.929 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.136 | 0.877 |
| Number of reflections | 39673 | 2068 |
| <I/σ(I)> | 15.7 | 2.2 |
| Completeness [%] | 94.9 | 97.4 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 8000, 0.1M CHES, pH 9.5 |
