6N6I

Human REXO2 bound to pGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	45.814, 87.289, 123.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.966 - 1.431
R-factor	0.1586
Rwork	0.158
R-free	0.17810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n6a
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.814	45.814	1.510
High resolution limit [Å]	1.431	4.530	1.431
Rmerge		0.040	0.858
Rmeas	0.107	0.043	0.945
Rpim	0.038	0.015	0.387
Total number of observations	691974
Number of reflections	91458	3190	12470
<I/σ(I)>	13.2	13.9	0.9
Completeness [%]	99.0	99.8	94
Redundancy	7.6	7.6	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.2 M sodium malonate (pH 5.5) and 15-20% polyethylene glycol 3350, 25% gylcerol