6N64

Crystal structure of mouse SMCHD1 hinge domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 41 21 2
Unit cell lengths	123.549, 123.549, 232.869
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.450 - 3.300
R-factor	0.206
Rwork	0.204
R-free	0.23900
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.450	3.418
High resolution limit [Å]	3.300	3.300
Rmeas	0.205	1.956
Rpim	0.048	0.461
Number of reflections	27936	2722
<I/σ(I)>	14.81	2.03
Completeness [%]	99.9	99.71
Redundancy	17.7	17.9
CC(1/2)	0.999	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.35	281	0.1 M Sodium Bicine pH 9.35, 0.18 M MgCl2, 20% (v/v) 2-propanol