6N5X

Crystal structure of the SNX5 PX domain in complex with the CI-MPR (space group P212121 - Form 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97803
Spacegroup name	P 21 21 21
Unit cell lengths	48.653, 64.730, 69.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.289 - 2.051
R-factor	0.2021
Rwork	0.200
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.654
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.110
High resolution limit [Å]	2.050	2.050
Number of reflections	14255
<I/σ(I)>	15.9
Completeness [%]	99.7
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	75% of 0.2 M (NH4)SO4, 0.1 M MES pH 6.5, 30% (w/v) PEG5000 MME + 15% glycerol + 40% v/v Polypropylene glycol P400