6N54

Crystal structure of human uridine-cytidine kinase 2 complexed with 2'-azidocytidine monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	198
Detector technology	CCD
Collection date	2018-04-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	F 2 2 2
Unit cell lengths	90.329, 141.263, 247.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.583 - 2.424
R-factor	0.1914
Rwork	0.189
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uei
RMSD bond length	0.003
RMSD bond angle	0.517
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmeas	0.105
Rpim	0.040	0.437
Number of reflections	29839	1456
<I/σ(I)>	31.9	1.66
Completeness [%]	99.9	99.7
Redundancy	7	6.5
CC(1/2)	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M Na/K phosphate, 20% PEG 3350