6N54
Crystal structure of human uridine-cytidine kinase 2 complexed with 2'-azidocytidine monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 198 |
| Detector technology | CCD |
| Collection date | 2018-04-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 90.329, 141.263, 247.887 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.583 - 2.424 |
| R-factor | 0.1914 |
| Rwork | 0.189 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uei |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.105 | |
| Rpim | 0.040 | 0.437 |
| Number of reflections | 29839 | 1456 |
| <I/σ(I)> | 31.9 | 1.66 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7 | 6.5 |
| CC(1/2) | 0.999 | 0.724 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Na/K phosphate, 20% PEG 3350 |
