6N4I
Structural basis of Nav1.7 inhibition by a gating-modifier spider toxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 221.220, 123.530, 123.990 |
| Unit cell angles | 90.00, 124.00, 90.00 |
Refinement procedure
| Resolution | 36.892 - 3.541 |
| R-factor | 0.2755 |
| Rwork | 0.275 |
| R-free | 0.29420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ek0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.854 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.115 | 0.760 |
| Number of reflections | 479752 | 35190 |
| <I/σ(I)> | 10.43 | |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 13.7 | 13.7 |
| CC(1/2) | 0.999 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 2.3-2.6M Ammonium sulfate, 100 mM HEPES, pH 7.0; 30% sucrose for cryo |
