6N44
Sheep Galectin-11 (LGALS11) complex with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.170, 127.450, 141.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.384 - 2.000 |
| R-factor | 0.1946 |
| Rwork | 0.192 |
| R-free | 0.23820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g86 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10_2148) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.384 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 115747 | |
| <I/σ(I)> | 1.33 | |
| Completeness [%] | 98.3 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2% v/v Tacsimate, pH 7.0, 0.1 M HEPES, pH 7.5, 20% w/v PEG3350 |
