6N3X

Human Histidine Triad Nucleotide Binding Protein 1 (Hint1) with Bound 5'-O-[1-Benzyl]Carbamoyl Guanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-09-24
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	77.877, 46.410, 64.032
Unit cell angles	90.00, 94.46, 90.00

Refinement procedure

Resolution	31.919 - 1.100
R-factor	0.1628
Rwork	0.162
R-free	0.17340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw2
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.919	31.919	1.160
High resolution limit [Å]	1.100	3.480	1.100
Rmerge		0.024	0.333
Rmeas	0.061	0.029	0.398
Rpim	0.032	0.016	0.215
Total number of observations	316410	9182	43008
Number of reflections	90215	2722	12885
<I/σ(I)>	15.7	45.7	3.2
Completeness [%]	97.7	90.6	96
Redundancy	3.5	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.3	293	100 mM MES, 35% PEG 8000