6N3D
Structure of HIV Tat-specific factor 1 U2AF Homology Motif (APO-State)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-19 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.8265 |
Spacegroup name | P 43 |
Unit cell lengths | 30.201, 30.201, 98.742 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.200 | 1.170 |
High resolution limit [Å] | 1.130 | 1.130 |
Rmerge | 0.054 | 0.252 |
Rpim | 0.033 | 0.252 |
Number of reflections | 61046 | 1696 |
<I/σ(I)> | 13.6 | 2.9 |
Completeness [%] | 98.8 | 99.6 |
Redundancy | 8.7 | 1.9 |
CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 15% PEG 8000, 0.4 M MgCl2, 0.1 M TRIS |