6N2E
Crystal Structure of Human Protocadherin-15 EC1-3 G16D N369D Q370N and Mouse Cadherin-23 EC1-2 T15E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.792, 65.397, 190.015 |
| Unit cell angles | 90.00, 99.12, 90.00 |
Refinement procedure
| Resolution | 49.520 - 2.900 |
| R-factor | 0.22605 |
| Rwork | 0.225 |
| R-free | 0.25332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mfo 4aq8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.639 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.115 | |
| Rpim | 0.055 | 0.236 |
| Number of reflections | 41847 | 1806 |
| <I/σ(I)> | 12.33 | |
| Completeness [%] | 93.2 | 86.5 |
| Redundancy | 5.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Imidazole pH 6.8, 46% MPD |
