6N2A
Meso-Diaminopimelate Decarboxylase from Arabidopsis thaliana (Isoform 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-08-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 80.968, 88.624, 121.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.660 - 1.880 |
| R-factor | 0.1717 |
| Rwork | 0.170 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p3e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.573 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.620 | 42.620 | 1.920 |
| High resolution limit [Å] | 1.880 | 9.010 | 1.880 |
| Rmerge | 0.147 | 0.029 | 1.301 |
| Rmeas | 0.158 | 0.031 | 1.407 |
| Rpim | 0.058 | 0.012 | 0.531 |
| Total number of observations | 525698 | 4874 | 31362 |
| Number of reflections | 71841 | 751 | 4528 |
| <I/σ(I)> | 9.6 | 27.5 | 1.5 |
| Completeness [%] | 99.9 | 99.3 | 98.7 |
| Redundancy | 7.3 | 6.5 | 6.9 |
| CC(1/2) | 0.997 | 0.999 | 0.678 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 8 mg/ml protein in buffer containing 0.2 mM PLP and 0.4 mM lysine mixed in a 1:1 ratio with a reservoir solution consisting of 0.2 M magnesium chloride, 0.1 M Tris pH 7.5, 20%(w/v) PEG 6000. |
